𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Rapid Screening Method to Monitor Expression of Recombinant Proteins from Various Prokaryotic and Eukaryotic Expression Systems Using Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry

✍ Scribed by Judith A. Jebanathirajah; Søren Andersen; Blagoy Blagoev; Peter Roepstorff

Book ID
102563166
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
118 KB
Volume
305
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Rapid methods using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry to monitor recombinant protein expression from various prokaryotic and eukaryotic cell culture systems were devised. Intracellular as well as secreted proteins from both induced and constitutive expression systems were measured and monitored from whole cells and growth media, thus providing an alternative to time-consuming traditional methods for screening and monitoring of protein expression. The methods described here involve minimal processing of samples and are therefore relevant to high-throughput screening applications.

📜 SIMILAR VOLUMES

Matrix-assisted Laser Desorption/Ionizat
Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry as a Rapid Screening Method to Detect Mutations Causing Tay–Sachs Disease
✍ Jannavi R. Srinivasan; Yan-hui Liu; Patrick J. Venta; David Siemieniak; Anthony 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 342 KB 👁 2 views

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been used as a rapid method for the detection of human genetic polymorphisms. In particular, the mutations in the human HEXA gene that cause the infantile Tay-Sachs disease have been studied using MALDI-MS to demonstrate th

Rapid profiling of E. coli proteins up t
Rapid profiling of E. coli proteins up to 500 kDa from whole cell lysates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
✍ Bathsheba E. Chong; Daniel B. Wall; David M. Lubman; Shannon J. Flynn 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 193 KB 👁 2 views

## Matrix -assisted laser desorption/ionization time-of-flight mass spectrometry was used to rapidly detect and profile large proteins from Escherichia coli whole cell lysates in the mass range 25-500 kDa. The bacterial samples were treated with guanidine hydrochloride and Triton X-100 to disrupt a

Web-based two-dimensional database of Sa
Web-based two-dimensional database of Saccharomyces cerevisiae proteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry
✍ Robert Wildgruber; Gerold Reil; Oliver Drews; Harun Parlar; Angelika Görg 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 139 KB 👁 1 views

An image based two-dimensional (2-D) reference map of very alkaline yeast cell proteins was established by using immobilized pH gradients (IPG) up to pH 12 (IPG 6-12, IPG 9-12 and IPG 10-12) for 2-D electrophoresis and by using matrix-assisted laser desorption/ionization-time of flight mass spectrom

Proteomic analysis of differential prote
Proteomic analysis of differential protein expression in a human hepatoma revertant cell line by using an improved two-dimensional electrophoresis procedure combined with matrix assisted laser desorption/ionization-time of flight-mass spectrometry
✍ Quan Yuan; Jie An; Ding-gan Liu; Li Sun; Ying-zi Ge; Ying-ling Huang; Gen-jun Xu 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 364 KB 👁 1 views

## Abstract The proteomic profiles of a human hepatoma revertant, CL1, and its original cell line, SMMC7721, were compared by using an improved two‐dimensional electrophoresis (2‐DE) procedure, with multi‐IPGstrips gels (length ≤ 13 cm) run simultaneously on one sodium dodecyl sulfate (SDS) gel (sh