A rapid reaction releasing the carboxyl terminal residues of peptides
β Scribed by Shiroki Yagisawa; Masako Urakami
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- French
- Weight
- 250 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0040-4039
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π SIMILAR VOLUMES
Proteins and peptides are digested with anhydrous hydrazine and the free amino acids, released from their carboxyl termini, are analyzed by reverse-phase chromatography as phenylthiocarbamyl (PTC) derivatives. Most of the potentially interfering hydrazides are removed due to the insolubility of thei
Ionspray ionization of solutions containing peptides (M) plus alkali metal ions X+ produces ( M + XI +, [ M + H + XI \*+ and [ M + 2x1 \*+ ions. Low-energy collision-induced dissociation of these species demonstrated a marked trend towards decreased strength of the M-X+ interaction with increasing s
The N-terminus of ristocetin A and ristomycin A was found to be the p-hydroxyphenylglycine ring of ristomycinic acid by reductive alkylation of the peptide with acetone and NaCNBH3 followed by peptide hydrolysis and separation of the two rings of the isopropyl amino acid by hydrogenolysis. Ristocet