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A Biocompatible Condensation Reaction for the Labeling of Terminal Cysteine Residues on Proteins

✍ Scribed by Hongjun Ren; Fei Xiao; Ke Zhan; Young-Pil Kim; Hexin Xie; Zuyong Xia; Jianghong Rao

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
606 KB
Volume
121
Category
Article
ISSN
0044-8249

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Proteins and peptides are digested with anhydrous hydrazine and the free amino acids, released from their carboxyl termini, are analyzed by reverse-phase chromatography as phenylthiocarbamyl (PTC) derivatives. Most of the potentially interfering hydrazides are removed due to the insolubility of thei