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A rapid purification procedure for tetrodotoxin derivatives by high-performance liquid chromatography

✍ Scribed by Takashi Nakayama; Susumu Terakawa

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 218 KB
Volume: 126
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90121-x

No coin nor oath required. For personal study only.

✦ Synopsis

A simple procedure for purification of tetrodotoxin (TTX) derivatives by high-performance liquid chromatography is described. Chemically oxidized TTX, Cl,-nortetrodotoxin (nor-TTX), was purified and collected by reverse-phase chromatography. The separation of nor-TTX from unreacted TTX was excellent and recovery of nor-T-TX was more than 90%. The isolated nor-TTX was further coupled with lysine, and the coupled product was purified again by high-performance liquid chromatography on a cation-exchange column. The separation of all compounds required less than 15 min. The uv monitoring at 230 nm allowed the detection of TTX derivatives at the 2-to 3-ng level.

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