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A rapid purification of T4 polynucleotide kinase using blue dextran-sepharose chromatography

โœ Scribed by Brian P. Nichols; Terry D. Lindell; Earle Stellwagen; John E. Donelson

Book ID
115734849
Publisher
Elsevier Science
Year
1978
Weight
503 KB
Volume
526
Category
Article
ISSN
0005-2744

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Rapid large-scale purification of pig he
Rapid large-scale purification of pig heart nucleoside diphosphate kinase by affinity chromatography on Cibacron Blue 3G-A Sepharose
โœ I. Lascu; M. Duc; A. Cristea ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 436 KB

A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography