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A rapid purification of hepatic ATP citrate lyase using blue sepharose

✍ Scribed by Jane C. Redshaw; Ernest G. Loten


Book ID
115912064
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
335 KB
Volume
123
Category
Article
ISSN
0014-5793

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Rapid large-scale purification of pig he
✍ I. Lascu; M. Duc; A. Cristea πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 436 KB

A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography