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Rapid purification of a chloroplast nucleoside diphosphate kinase using CoA-affinity chromatography

✍ Scribed by Li-Ming Yang; Gayle K. Lamppa


Book ID
113269828
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
544 KB
Volume
1294
Category
Article
ISSN
0167-4838

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A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography