A rapid method for separation and identification of several hexuronic acids and hexuronic acid-containing oligosaccharides

✍ Scribed by Masashi Kosakai; Zensaku Yosizawa

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 276 KB
• Volume: 69
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90144-x

✦ Synopsis

Five naturally occurring hexuronic acids and several hexuronic acid-containing oligosaccharides were separated and identified by high voltage paper electrophoresis, using one of the following buffers. (i) Pyridine-acetic acid-water (1: 10:89, by volume), the pH of which was adjusted to 2.3-3.5 with 98% formic acid. (ii) Pyridine-water

(1:90, by volume), the pH of which was adjusted to 3.5-4.0 with glacial acetic acid. The best separation of the five hexuronic acids and heparin disaccharides was observed at pH 2.7 after electrophoresis for 180 min at 100 V/cm. At pH 3 L-gulosyluronic acid-L-guluronic acid could be easily isolated from an acid hydrolysate of alginate by the present method.