A simple and rapid electrophoretic method for the separation of glucuronic acid and iduronic acid

✍ Scribed by Itaru Miyamoto; Sumi Nagase

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 491 KB
• Volume: 115
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90010-5

✦ Synopsis

A new electrophoretic method using Titan III cellulose acetate plates has been developed for the separation and quantitation of glucuronic acid and iduronic acid. This method is quite simple, and glucuronic acid and iduronic acid can be separated within 50 min. This method was applied to the analyses of uranic acids in chondroitin sulfates A and C, and dermatan sulfate.

Uranic acids are major structural components of glycosaminoglycans from mammalian tissues. Hybrid structures of glucuranic acid and iduronic acid were found in dermation sulfate (DS),2 heparin, and heparan sulfate. Gas chromatographic methods ( 1,2) or ion-exchange chromatographic methods (3-5) were commonly used for the determination of uranic acids. However, these methods are rather complicated and require expensive equipment.

In this report we describe a simple and rapid electrophoretic method for the separation and quantitation of glucuronic acid and iduronic acid using Titan III cellulose acetate plates.

MATERIALS AND METHODS

Materials.

Chondroitin sulfate A (ChS-A) from whale cartilage, chondroitin sulfate C (ChS-C) from shark cartilage, and DS from pig skin were obtained from Seikagaku Kogyo Company, Tokyo, Japan. L-Iduronic acid was a gift from Dr. H. Kushida, Ika-