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A rapid method for determining soot content of lungs in diesel-exposed rodents

✍ Scribed by Rogene F Henderson; James J. Waide; Joe L. Mauderly; Roger O. McClellan

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
312 KB
Volume
7
Category
Article
ISSN
0260-437X

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✦ Synopsis

A rapid optical method for determining the quantity of soot in the lungs of rodents exposed to diluted diesel exhaust has been developed. The method is nondestructive to enzymatic components of the lung, allowing dose and biochemical assays to be done on the same sample. The lung burdens of soot were determined by comparing the extinction of light at 620 nm of lung homogenates from exposed animals to that of lung homogenates containing spiked standards.

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