Methods for the detection of RNase after polyacrylamide gel electrophoresis have required long incubation times or indirect techniques that allowed the enzyme ample time to diffuse into a broad band (l-3). Wolf (4) recently described a new technique in which the gel was incubated in a solution of lo
A rapid laboratory method for developing contact prints of stained polyacrylamide gels
β Scribed by Dr. Bohn D. Dunbar; Donnie S. Bundman; Bonnie S. Dunbar
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 197 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0173-0835
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A simple method for protein recovery from gel slices following polyacrylamide gel electrophoresis is proposed which requires neither additional devices nor much skill. The method is based on reversed electrophoresis using a discontinuous conductivity gradient which is in turn maintained by a gradien