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A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass

✍ Scribed by G. Braunegg; B. Sonnleitner; R. M. Lafferty

Publisher
Springer
Year
1978
Tongue
English
Weight
446 KB
Volume
6
Category
Article
ISSN
1432-0614

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✦ Synopsis

The gas chromatographic method for the determination of poly-/3-hydroxybutyric acid (PHB) consists of a mild acid or alkaline methanolysis of poly-~-hydroxybutyric acid directly without previous extraction of PHB from the cells; this is followed by gas chromatography of the 3-hydroxybutyric acid methylester. The method is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10 -5 g/1. Only 4 h is required from sampling from the fermenter till completion of the PHB determination.

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