A rapid batch assay for cyclic AMP phosphodiesterase
โ Scribed by David R. Zusman
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 848 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A procedure for the assay of cyclic nucleotide phosphodiesterase is described in which labeled cyclic nucleotide is separated from labeled nucleoside by the batchwise addition of ethanolic slurries of Dowex 2 fluoride. Under the conditions described there is no detectable adsorption of nucleoside by
A new method for assay of cyclic AMP phosphodiesterase (EC 3.1.4.17) has been developed based on the observation that a mixture of cyclic AMP and AMP can be resolved on a column of florisil (activated magnesium silicate) at pH 7.0. The cyclic nucleotide is retained by the silicate and the AMP which
The search for cyclic nucleotide phosphodiesterase inhibitors in large chemical and natural product libraries is limited by assay throughput. A high-throughput assay that can monitor different phosphodiesterase activities would be useful for these inhibitor searches. We have developed a sensitive ph