A High-Throughput Assay for Cyclic Nucleotide Phosphodiesterases

✍ Scribed by R.J. Schilling; D.R. Morgan; B.F. Kilpatrick

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 380 KB
• Volume: 216
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1019

✦ Synopsis

The search for cyclic nucleotide phosphodiesterase inhibitors in large chemical and natural product libraries is limited by assay throughput. A high-throughput assay that can monitor different phosphodiesterase activities would be useful for these inhibitor searches. We have developed a sensitive phosphodiesterase assay which is based on the selective precipitation of the radiolabeled 5'-nucleotide product by zinc and barium. This assay is unique to previous precipitation methods in that it measures product formed, either GMP or AMP. The procedure was adapted to a microtiter plate format for high-throughput, making it possible to perform in excess of 960 assays per day. This method was used to determine accurate enzyme kinetic constants for three different phosphodiesterases. The results agree favorably with literature values. Using the cyclic guanosine 3',5'-monophosphate-specific phosphodiesterase, IC50 values for the inhibitors M&B 22,948 and 3-isobutyl-1-methylxanthine were also determined. Demonstration of appropriate Km and inhibitor IC50 values verifies the ability of this new assay to identify selective cyclic nucleotide phosphodiesterase inhibitors.