A modified cyclic AMP binding assay
โ Scribed by W.David Lust; Ernestine Dye; Ann V. Deaton; Janet V. Passonneau
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 467 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Human erythrocyte ghosts are convenient for use in cyclic AMP binding assays in the picomole range. Extraction of the membranes with NH4C1 immediately before use eliminates interference by ATP, which occurs with native membranes. The erythrocyte preparation has high affinity for several N 6 and C-8
The radioisotope dilution test for cyclic AMP with binding protein from beef muscle can be described mathematically with the assumption that one molecule of binding protein binds one molecule of cyclic AMP. Formulas for the dose response under saturation and nonsaturation conditions are given. Quasi