โ Scribed by Joanne E. Hesse; Lucia B. Rothman-Denes; Wolfgang Epstein
No coin nor oath required. For personal study only.
Human erythrocyte ghosts are convenient for use in cyclic AMP binding assays in the picomole range. Extraction of the membranes with NH4C1 immediately before use eliminates interference by ATP, which occurs with native membranes. The erythrocyte preparation has high affinity for several N 6 and C-8 derivatives of cyclic AMP, moderate affinity for cyclic IMP, and weak but significant affinity for several other 3'-5'-cyclic nucleotides.
We wish to bring to general attention a cyclic AMP binding assay utilizing human erythrocyte ghosts. This assay material is easy to prepare, stable to storage, and has a sensitivity similar to that of assays with soluble protein kinases. The erythrocyte assay is subject to interference by ATP, but this effect is eliminated by salt extraction of the membranes.
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๐ SIMILAR VOLUMES
The radioisotope dilution test for cyclic AMP with binding protein from beef muscle can be described mathematically with the assumption that one molecule of binding protein binds one molecule of cyclic AMP. Formulas for the dose response under saturation and nonsaturation conditions are given. Quasi