✦ LIBER ✦

A rapid assay for protein kinases phosphorylating small polypeptides and other substrates

✍ Scribed by S. Braun; M.Abdel Ghany; E. Racker

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 831 KB
Volume: 135
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90698-x

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✦ Synopsis

A new and rapid method of protein kinase assay is presented which is suitable for low-molecular-weight substrates, irrespective of their electrophoretic or chromatographic mobility. It depends on the phosphorylation of the substrates with [gamma-32P]ATP, hydrolysis of the pyrophosphate bonds by boiling in 1 N HCl, extraction of 32P with isobutanol-benzene, and measurement of the radioactivity of 32P-labeled phosphoesters in the water phase. The method is shown to be suitable for both tyrosine- and serine-phosphorylating protein kinases.

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