The assay of acidic peptides as substrates for protein kinases has not been as easy to perform as testing basic peptides or polypeptides. We have developed a simple, rapid, and cost-effective procedure that allows the design and testing of potential peptide substrates without the constraints imposed
A Capillary Electrophoresis-Based Assay for Protein Kinases and Protein Phosphatases Using Peptide Substrates
β Scribed by J.F. Dawson; M.P. Boland; C.F.B. Holmes
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 532 KB
- Volume
- 220
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Capillary electroseparations of enkephalin-related peptides and protein kinase A peptide substrates The separations of enkephalin-related peptides and protein kinase A peptide substrates, with the common structural feature -Arg-Arg-X-Ser-Val-, were studied in micellar electrokinetic chromatogra
The recently described synthesis of ferric adsorbent paper has made possible the modification of protein kinase assays. The adsorbent contains ferric chelate groups, which are responsible for the binding of phosphopeptide via phosphate group. The selective adsorption of phosphopeptide contra nonphos
A method was devised for assaying protein kinases that phosphorylate either Kemptide, such as cAMP-dependent protein kinase, or a glycogen synthase peptide, which is an excellent substrate for protein kinase C. Upon sequential processing of reaction mixtures through tandem columns of cation and anio