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A rapid and sensitive method for studying the binding of ribonucleotide polymers to mammalian ribosomal subunits

✍ Scribed by L. Lim

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
573 KB
Volume
69
Category
Article
ISSN
0003-2697

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✦ Synopsis

A sensitive and rapid method has been developed for studying the interactions of ribonucleotide homopolymers with isolated liver ribosomal subunits. Small amounts of ribosomal subunits are first immobilised on Millipore filters. The homopolymers are then allowed to interact with the ribosomes by slow passage through the filters. Conditions are described under which both the large and the small subunits can bind poly(A) and poly(U) as well as poly(G). The poly(A) and poly(G) binding sites can be shown to be different.

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