A rapid and sensitive high-performance liquid chromatographic assay for 6-mercaptopurine metabolites in red blood cells

✍ Scribed by Lawrence E. Lavi; John S. Holcenberg

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 635 KB
• Volume: 144
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90148-4

✦ Synopsis

A highly sensitive and rapid assay for the detection of 6-mercaptopurine metabolites in the red blood cells-of leukemic patients receiving the drug has been developed. The method employs a batchchromatographic procedure using a mercurial cellulose resin to selectively absorb thiol compounds combined with separation by high-performance liquid chromatography using a Partisil-SAX column and uv detection. This method permits detection of 6-thioinosine monophosphate, 6-thiouric acid, and 6-thioguanosine mono-, di-, and triphosphates in patient samples with a sensitivity of 5-10 pmol. No 6-thioinosine di-or triphosphates were detected in patient samples. The results of our study indicate that 6-thioguanosine triphosphate is the major metabolite of 6-mercaptopurine retained by red blood cells after oral or iv administration Of the drug.