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A rapid and sensitive detection of proteolytic enzymes after electrophoresis

โœ Scribed by B. Dahlmann; K.L.D. Jany


Book ID
108335332
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
265 KB
Volume
110
Category
Article
ISSN
1873-3778

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A sensitive, microplate assay is described for the detection of a wide range of proteolytic enzymes, using radio-iodine-labeled gelatin as substrate. The technique uses the Bolton-Hunter reagent to label the substrate, which is then coated onto the wells of polyvinyl chloride microtiter plates. By m