Rapid detection of proteolytic enzymes and their inhibitors
✍ Scribed by Ivo Šafařik
- Publisher
- Springer-Verlag
- Year
- 1989
- Tongue
- English
- Weight
- 91 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0951-208X
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The pattern of synonymous and nonsynonymous substitutions at the reactive center of proteases (kallikrein) and their inhibitors (ctt-antitrypsin and serpin) was examined. In the case of Cta-antitrypsin, the proportion of different nonsynonymous sites exceeds that of different synonymous sites at the
A very sensitive radioisotope method, using 1251-labelled serum albumin as substrate, is proposed for the detection of very low enzymatic activities released from insoluble matrix to eluate, storage media and washings after an immobilization procedure. In the case of papain, proteolytic activities