✦ LIBER ✦

A radioisotopic method for the assay of aspartate carbamoyltransferase and carbamoyl phosphate

✍ Scribed by Richard I. Christopherson; Lloyd R. Finch

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 807 KB
Volume: 73
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90179-2

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✦ Synopsis

The use of [14C]aspartate of high specific activity and thin-layer chromatography on polyethyleneimine cellulose for the separation of carbamoyl aspartate from aspartate has enabled the measurement of aspartate carbamoyltransferase and carbamoyl phosphate synthase activities and carbamoyl phosphate concentrations in extracts from Escherichia coli. The assay method described is sensitive to the formation of about 1 pmol of carbamoyl aspartate.

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