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A radioimmunoassay for the determination of tipredane in human plasma

✍ Scribed by M.A.J. Bayliss; J.R. Preston; D. Wilkinson; D.J. Wilkinson; W.J.S. Lockley

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 648 KB
Volume: 15
Category: Article
ISSN: 0731-7085
DOI: 10.1016/0731-7085(96)01816-x

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✦ Synopsis

A sensitive method for the determination of tipredane in human plasma has been developed. Prior to radioimmunoassay, proteins are removed from plasma by precipitation with acetonitrile. A [125I]iodohistamide derivative of tipredane is used as a heterogeneous radioligand. The primary antiserum is raised in sheep against a tipredane (O-carboxymethyl) oxime-bovine serum albumin conjugate. Donkey anti-sheep IgG antiserum is used as the secondary antiserum in a double antibody separation procedure. The limit of quantification of the method is 1 ng ml-1 for 500 microliters of plasma. There is no significant interference from either established or putative metabolites of tipredane, endogenous steroids or a wide range of other drugs. The assay was used to determine the concentrations of tipredane in plasma samples collected in pilot clinical studies.

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