𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Protein Biosensor for Lactate

✍ Scribed by Sabato D'Auria; Zygmunt Gryczynski; Ignacy Gryczynski; Mosé Rossi; Joseph R. Lakowicz

Book ID
102562966
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
98 KB
Volume
283
Category
Article
ISSN
0003-2697

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✦ Synopsis

Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for L-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactatesensing protein.

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