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Amperometric biosensor for glucose and lactate

✍ Scribed by A. Kaisheva; P. Atanasov; S. Gamburzev; N. Dimcheva; I. Iliev

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
509 KB
Volume
8
Category
Article
ISSN
0925-4005

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✦ Synopsis

Amperometric membrane glucose and lactate biosensors are investigated. 'Sandwich'-type membranes are used with an enzyme (glucose oxidase or lactate oxidase) immobilized between two membrane layers. A new type of catalytic electrode made from pyrolysed CoTMPP (cobalt-tetramethoxyphenylporphyrin) is used for detection of H,Oz generated by the enzymatic reaction. The glucose biosensor is used for the determination of glucose concentrations up to 2 mM/l in water solutions and in diluted blood serum samples. The biosensor can be used for more than 100 days. Concentrations of Li-lactate up to 1.5 mM/l in water solutions are determined with the lactate biosensor.

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