A progestin effect on lactate dehydrogenase in the human breast cancer cell line T-47D
β Scribed by Rodney D. Hagley; Michael R. Moore
- Book ID
- 115758172
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 295 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0006-291X
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## Abstract Steroid regulation of 17Ξ²βhydroxysteroid dehydrogenase (17βHSD) was studied in the Tβ47D human breastβcancer cell line, using a radioimmunoassay. In addition, 3 mRNA species (2.4, 1.4, and 0.9 kb) specific for the enzyme were shown to be present in these cells. All the synthetic progest
## CHEMISTS 2h to complete, is precise (CV's<I0%) and highly sensitive, with a detection limit of <0.05 pg/L. Comparison with an enzymatic CK-BB assay for 166 breast tumor cytosols (determination of CK-B subunit after immunoinhibition of CK-M subunit) gave the following equation: y (TR-FIA method)