Androgens, progestins and tamoxifen induce PSA production in the breast cancer cell line T-47D

✍ Scribed by E.P. Diamandis; Y. He; L. Grass

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 124 KB
• Volume: 27
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(94)90153-8

✦ Synopsis

CHEMISTS

2h to complete, is precise (CV's<I0%) and highly sensitive, with a detection limit of <0.05 pg/L. Comparison with an enzymatic CK-BB assay for 166 breast tumor cytosols (determination of CK-B subunit after immunoinhibition of CK-M subunit) gave the following equation: y (TR-FIA method) = 0.71 x (enzymatic method) + 5,8, r = 0.83. Crossreactivity of the CK-BB TR-FIA assay by CK-MM was not detectable at concentrations as high as 1000 pg/L. The cross-reactivity of CK-MB isoenzyme was 5% at 100 pg/L of CK-MB. The method was used to measure CK-BB activity in 166 breast tumor cytosols which were also measured for (ER) and progesterone (PR) receptors. Using a cutoff value of 10 fmol/mg protein for the receptors and 26 pg/L for CK-BB, we examined the possible association between receptors and CK-BB. We found a strong association between CK-BB and ER (P=0.001, chi-square test) and a weak association between CK-BB and PR (P=0.054). We conclude that CK-BB production by breast tumors is mediated by the action of the estrogen receptor.