A simplified procedure for the synthesis of [beta-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commercially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an ext
A procedure for the synthesis of uridine-5′-diphospho-N-acetylglucosamine-14C
✍ Scribed by Ronald R. Wagner; Morris A. Cynkin
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 377 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
Galacturonic acid (GalA) is a major component of plant cell-wall-derived pectins. It can be also found in the cell-surface polysaccharides of different microorganisms, including several symbiotic and pathogenic bacteria. Uridine diphosphogalacturonic acid (UDP-GalA) is a likely donor for GalA during
MATERIALS AND METHODS ## Carboxy-p-amino[14C]benzoic acid was obtained from ICN and from Research Products International. Thionyl chloride was obtained from Matheson, Coleman and Bell, adenosine was obtained from Sigma, and hexamethylphosphoramide was obtained from Aldrich. Thin-layer chromatogr
Many plant cell wall components such as the polysaccharides xylans and pectins or the glycoproteins arabinogalactan proteins and extensins contain arabinosyl residues. The arabinosyl substituents are thought to be incorporated into these wall polymers by the action of arabinosyltransferases using UD