✦ LIBER ✦
A simplified procedure for synthesizing large quantities of highly purified uridine [β-32P]diphospho-N-acetylglucosamine
✍ Scribed by Les Lang; Stuart Kornefeld
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 465 KB
- Volume
- 140
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A simplified procedure for the synthesis of [beta-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commercially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.