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A procedure for the preparation of [32P]phosphatidic acid

✍ Scribed by R.Kennedy Keller; W.Lee Adair; Nancy Cafmeyer

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 546 KB
Volume: 155
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90235-6

No coin nor oath required. For personal study only.

✦ Synopsis

The phosphorylation procedure of F. Cramer, W. Rittersdorf, and W. Bohm [(1961) Chem. Ber. 654, 180] using bis(triethylammonium) phosphate and trichloroacetonitrile was shown to be effective in the synthesis of [32P]phosphatidic acid. From diacylglyceride and 0.5 mCi H(3)32PO4, 25-50 microCi of labeled material (sp act = 1 mCi/mumol) can be prepared in 2 h. The product was shown to be radiochemically pure by both TLC and HPLC. L- and DL-[32P]dipalmitoyl phosphatidic acid prepared using this procedure were shown to be hydrolyzed by rat liver microsomes at approximately the same rates.

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