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A potentiometric method for the determination of the iodine-binding capacity of glycogen

✍ Scribed by Frank W. Fales

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 635 KB
Volume: 132
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90030-1

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✦ Synopsis

The experimental conditions in the potentiometric method for the determination of the iodinebinding capacity (I,,) of starch and amylose [R. L. Bates, D. French, and R. E. Rundle (1943) J. Amer. Chem. Sot. 65, 142-1481 were not suitable for glycogen because of the much lower affinity for iodine of the latter. This difficulty was overcome by titration of small volume with both the iodine and glycogen at high concentration. Using the concentration cell circuit Pt electrode-blank-bridge-glycogen-Pt electrode, small increments of standard iodine solution were added to the blank solution and each was titrated to null by adding iodine to the glucogen solution [G. A.

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