## Abstract A screening procedure based on the formation of lactoseโfermenting papillae on lactoseโnonfermenting bacterial colonies has been described. Due to supplementation of 50 ฮผg/ml 2โaminopurine in EMB plates the frequency of papillae rose to an average number of 28 papillae per colony. Of 15
A potential method for selecting genetic deletions in Escherichia coli
โ Scribed by Burdon, Michael G.
- Book ID
- 104703801
- Publisher
- Springer
- Year
- 1970
- Tongue
- English
- Weight
- 190 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0026-8925
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A mutation (lspA, prolipoprotein signal peptidase) rendering the prolipoprotein signal peptidase temperature-sensitive in Escherichia coli has been analyzed. The mutation was mapped in the dnaJ-rpsT-ileS-dapB region by interrupted mating with various Hfr strains and P1 phage transduction. lambda tra
## Abstract The influence of linker design on fusion protein production and performance was evaluated when a family 9 carbohydrateโbinding module (CBM9) serves as the affinity tag for recombinant proteins expressed in __Escherichia coli__. Two bioinformatic strategies for linker design were applied