✦ LIBER ✦

Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli

✍ Scribed by Yamagata, Hideo ;Taguchi, Nana ;Daishima, Kyoko ;Mizushima, Shoji

Publisher: Springer
Year: 1983
Tongue: English
Weight: 499 KB
Volume: 192
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00327640

No coin nor oath required. For personal study only.

✦ Synopsis

A mutation (lspA, prolipoprotein signal peptidase) rendering the prolipoprotein signal peptidase temperature-sensitive in Escherichia coli has been analyzed. The mutation was mapped in the dnaJ-rpsT-ileS-dapB region by interrupted mating with various Hfr strains and P1 phage transduction. lambda transducing phage lambda ddapB2 that carries the rpsT-ileS-dapB region was shown to complement the lspA mutation. Plasmid pLC3-13 which had been isolated from Clarke and Carbon's collection as a plasmid carrying the lspA locus was shown to carry the dnaJ and rpsT loci. Complementation analysis with plasmids carrying various DNA fragments derived from pLC3-13 showed that the lspA locus is between the rpsT and ileS loci. The wildtype allele was dominant over the lspA allele.

📜 SIMILAR VOLUMES

A distinct signal peptidase for prolipop

A distinct signal peptidase for prolipoprotein in escherichia coli

✍ Masao Tokunaga; Judith M. Loranger; Henry C. Wu 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 475 KB

We have previously demonstrated the modification and processing of Escherichia coli prolipoprotein (Braun's) in vitro (Tokunaga M, Tokunaga H, Wu HC: Proc Natl Acad Sci USA 79:2255, 1982). Using this in vitro assay of prolipoprotein signal peptidase and globomycin selection, we have isolated and par

Genetic mapping of trxA, a gene affectin

Genetic mapping of trxA, a gene affecting thioredoxin in Escherichia coli K12

✍ Mark, David F. ;Chase, John W. ;Richardson, Charles C. 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 724 KB

Reversion of a truncated gene for ampici

Reversion of a truncated gene for ampicillin resistance by genetic rearrangements in Escherichia coli K12

✍ Iida, Shigeru ;Hiestand-Nauer, Rosemarie ;Hänni, Christine ;Arber, Werner 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 343 KB

The composite transposon Tn2672 is a derivative of the Tn3-related transposon Tn902 whose bla gene providing ampicillin resistance had been inactivated by the insertion of the IS1-flanked multiple drug resistance transposon Tn2671. Most ampicillin resistant revertants of Tn2672 are due to precise ex

The fim genes responsible for synthesis

The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli, cloning and genetic organization

✍ Klemm, Per ;Jørgensen, Birthe Jul ;van Die, Irma ;de Ree, Han ;Bergmans, Hans 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 546 KB

Genetic analysis of essential genes in t

Genetic analysis of essential genes in the ftsE region of the Escherichia coli genetic map and identification of a new cell division gene, ftsS

✍ Salmond, George P. C. ;Plakidou, Stella 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 548 KB

Several conditional lethal mutants of Escherichia coli have been analysed genetically using generalized transduction and lambda transducing vectors. Three temperature-sensitive ftsE mutants were found as was a cold-sensitive ftsE mutant. A new gene was found which mapped close to ftsE, namely ftsS.

Physical mapping of the gene for aminope

Physical mapping of the gene for aminopeptidase N in Escherichia coli K12

✍ Bally, M. ;Murgier, M. ;Tommassen, J. ;Lazdunski, A. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 139 KB

The pepN gene has been cloned into the multicopy plasmid pBR322. The restriction map of the insert was established and the gene was localized. By comparison with the restriction map of the plasmid pJP30 bearing the ompF region, it has been possible to order the ompF, asnS, and pepN genes. The ompF a