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A direct selection method for isolating antimutators of Escherichia coli

✍ Scribed by A. Quinones; Dr. R. Piechocki

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
268 KB
Volume
21
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

A screening procedure based on the formation of lactose‐fermenting papillae on lactose‐nonfermenting bacterial colonies has been described. Due to supplementation of 50 μg/ml 2‐aminopurine in EMB plates the frequency of papillae rose to an average number of 28 papillae per colony. Of 150,000 mutagenized colonies inspected 16 colonies were detected with drastically reduced or no papillae per colony.

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