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A possible error during assays for the enzymic phosphorylation of proteins and nucleic acids

✍ Scribed by P.J. Greenaway

Book ID
118853634
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
247 KB
Volume
47
Category
Article
ISSN
0006-291X

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Fluorescamine is a useful reagent in monitoring protein-DNA interactions only if a convenient method of separating the complex from free protein is available. Sedimentation of the complex provides such a method at least in the case of histone-like proteins capable of extensive interaction with DNA.