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A plasmid cloning vector with precisely regulatable copy number inEscherichia coli

✍ Scribed by Anna Herman-Antosiewicz; Michał Obuchowski; Grzegorz Węgrzyn

Book ID
111640171
Publisher
Humana Press Inc
Year
2001
Tongue
English
Weight
147 KB
Volume
17
Category
Article
ISSN
1073-6085

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## Animprovedvectorof2889bpwM construoted by mutation of copy number control system, into which f~geneswithoutarwiththestartcodanATGcaslbedirectfyinsatedforhigh-levelexpressjonin Ederichiu cd. Deletion of the rop gene mooding a negative regulation protein ROP leads to increase the plssmidcopynumbe