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An efficient plasmid vector for expression cloning of large numbers of PCR fragments inEscherichia coli

โœ Scribed by Christoph Reisinger; Alexander Kern; Kateryna Fesko; Helmut Schwab


Publisher
Springer
Year
2007
Tongue
English
Weight
146 KB
Volume
77
Category
Article
ISSN
1432-0614

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## Animprovedvectorof2889bpwM construoted by mutation of copy number control system, into which f~geneswithoutarwiththestartcodanATGcaslbedirectfyinsatedforhigh-levelexpressjonin Ederichiu cd. Deletion of the rop gene mooding a negative regulation protein ROP leads to increase the plssmidcopynumbe