✦ LIBER ✦

A Photochemical Switch for Controlling Protein–Protein Interactions

✍ Scribed by Sonia K. Pollitt; Peter G. Schultz

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 113 KB
Volume: 37
Category: Article
ISSN: 0044-8249
DOI: 10.1002/(sici)1521-3773(19980817)37:15<2104::aid-anie2104>3.0.co;2-z

No coin nor oath required. For personal study only.

✦ Synopsis

Incorporation of an unnatural amino acid containing a photolabile group in the side chain allows specific interactions between two proteins to be prevented. The photocaged ras protein in which Asp 38 has been substituted by its β-nitrobenzyl ester (Nb) is unable to interact with its effector protein p120-GAP (see drawing below) although it has the same intrinsic GTPase activity. After photocleavage of the Nb group, 50% of the p120-GAP-dependent GTPase activity relative to the wild-type protein is restored.

📜 SIMILAR VOLUMES

Allosteric Switches: Remote Controls for

Allosteric Switches: Remote Controls for Proteins

✍ Thorsten Berg 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB

Protein-Silicone Interactions

✍ Vasiliki Bartzoka; Mark R. McDermott; Michael A. Brook 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 115 KB 👁 2 views

Interaction potentials for protein foldi

Interaction potentials for protein folding

✍ Flavio Seno; Amos Maritan; Jayanth R. Banavar 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 56 KB 👁 1 views

We outline a general strategy for determining the effective coarse-grained interactions between the amino acids of a protein from the experimentally derived nativestate structures. The method is, in principle, free from any adjustable or empirically determined parameters, and it is tested on simple

Thermodynamic databases for proteins and

Thermodynamic databases for proteins and protein–nucleic acid interactions

✍ Akinori Sarai; M. Michael Gromiha; Jianghong An; Ponraj Prabakaran; Samuel Selva 📂 Article 📅 2001 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 464 KB

Protein-protein and protein-salt interac

Protein-protein and protein-salt interactions in aqueous protein solutions containing concentrated electrolytes

✍ R. A. Curtis; A. Montaser; J. M. Prausnitz; H. W. Blanch 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 7 KB 👁 2 views

Protein-protein and protein-salt interac

Protein-protein and protein-salt interactions in aqueous protein solutions containing concentrated electrolytes

✍ R. A. Curtis; J. M. Prausnitz; H. W. Blanch 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 167 KB 👁 2 views

Protein-protein and protein-salt interactions have been obtained for ovalbumin in solutions of ammonium sulfate and for lysozyme in solutions of ammonium sulfate, sodium chloride, potassium isothiocyanate, and potassium chloride. The two-body interactions between ovalbumin molecules in concentrated