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A partial reductive-cleavage study of the capsular polysaccharide of Escherichia coli K57

✍ Scribed by Haralambos Parolis; Shawn M.R. Stanley; Anne Dell; Andrew J. Reason

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 378 KB
Volume: 266
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(94)00242-8

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✦ Synopsis

Trideuteriomethylated and methylated derivatives of the capsular polysaccharide of Escherichia coli K57 were partially cleaved by Et3SiH, using Me3SiOSO2Me and Me3SiOSOeCF 3 as catalysts, to produce oligosaccharide-anhydroalditols. The structures of the trideuteriomethylated trisaccharide-and tetrasaccharide-anhydroalditols isolated were established by FABMS and NMR spectroscopy. Although conditions for the selective production of the tetrasaccharideanhydroalditol could not be established, oligosaccharide-anhydroalditols were isolated in sufficiently high yield to make this an attractive approach for the structural elucidation of the repeating units of bacterial polysaccharides.

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