A novel transposon-based method for elimination of large bacterial plasmids
✍ Scribed by Ariel Imre; Ferenc Olasz; János Kiss; Béla Nagy
- Book ID
- 113855259
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 484 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0147-619X
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A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15–40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca
A general method has been developed for the deletion of restriction endonuclease sites in bacterial plasmid DNA. The procedure involves partial digestion of the covalently closed circular plasmid DNA with an appropriate restriction endonuclease under conditions which allow accumulation of unit-lengt