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A Novel Strategy for Human Papillomavirus Detection and Genotyping with SybrGreen and Molecular Beacon Polymerase Chain Reaction

✍ Scribed by Károly Szuhai; Emily Sandhaus; Sandra M. Kolkman-Uljee; Marc Lemaître; Jean-Christophe Truffert; Roeland W. Dirks; Hans J. Tanke; Gert Jan Fleuren; Ed Schuuring; Anton K. Raap


Book ID
117021426
Publisher
American Society for Investigative Pathology
Year
2001
Tongue
English
Weight
198 KB
Volume
159
Category
Article
ISSN
0002-9440

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## Abstract The ability to detect the presence of human pap‐illomavirus (HPV)‐DNA sequences in urine was evaluated using polymerase chain reaction (PCR). DMA was purified and extracted from urine samples, and subjected to 40 cycles of amplification using the consensus primer pair MY11 and MY09. Coa