𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Molecular beacons: A real-time polymerase chain reaction assay for detecting Escherichia coli from fresh produce and water

✍ Scribed by S. Sandhya; Wilfred Chen; Ashok Mulchandani


Book ID
108047936
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
492 KB
Volume
614
Category
Article
ISSN
0003-2670

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Molecular Beacons: A Real-Time Polymeras
✍ Wilfred Chen; Grisselle Martinez; Ashok Mulchandani πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 146 KB

Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular bea

Use of Real-Time Polymerase Chain Reacti
✍ Nathalie Y. Fortin; Ashok Mulchandani; Wilfred Chen πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 87 KB

Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t