✦ LIBER ✦

Molecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Salmonella

✍ Scribed by Wilfred Chen; Grisselle Martinez; Ashok Mulchandani

Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 146 KB
Volume: 280
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.2000.4518

No coin nor oath required. For personal study only.

✦ Synopsis

Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular beacons were designed to recognize a 16-base-pair region on the amplicon. As few as 2 colony-forming unit (CFU) per PCR reaction could be detected. We also demonstrated the ability of the molecular beacons to discriminate between amplicons obtained from similar species such as Escherichia coli and Citrobacter freundii in realtime PCR assays. These assays could be carried out entirely in sealed PCR tubes, enabling fast and direct detection of Salmonella in a semiautomated format.

📜 SIMILAR VOLUMES

Immunopolymerase Chain Reaction Using Re

Immunopolymerase Chain Reaction Using Real-Time Polymerase Chain Reaction for Detection

✍ Paul W. Sims; Mark Vasser; Wai Lee Wong; P.Mickey Williams; Y.Gloria Meng 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 56 KB

All articles available online at http://www.idealibrary.com on FIG. 3. Correlation of VEGF concentrations in 40 normal human serum samples measured by fluorometric ELISA ( y axis) and immuno-PCR ( x axis) ( y ϭ 0.76 x Ϫ 7 pg/ml, correlation coefficient ϭ 0.83, P Ͻ 0.0001).

Use of Real-Time Polymerase Chain Reacti

Use of Real-Time Polymerase Chain Reaction and Molecular Beacons for the Detection of Escherichia coli O157:H7

✍ Nathalie Y. Fortin; Ashok Mulchandani; Wilfred Chen 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 87 KB

Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t

A Molecular Beacon for Quantitative Moni

A Molecular Beacon for Quantitative Monitoring of the DNA Polymerase Reaction in Real-Time

✍ Daniel Summerer; Andreas Marx 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 90 KB 👁 1 views

Problem-solving test: Real-time polymera

Problem-solving test: Real-time polymerase chain reaction

✍ József Szeberényi 📂 Article 📅 2009 🏛 The American Society for Biochemistry and Molecula 🌐 English ⚖ 53 KB 👁 1 views

A New Quantitative Method of Real Time R

A New Quantitative Method of Real Time Reverse Transcription Polymerase Chain Reaction Assay Based on Simulation of Polymerase Chain Reaction Kinetics

✍ Weihong Liu; David A. Saint 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 108 KB

Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference

Silicon inhibition effects on the polyme

Silicon inhibition effects on the polymerase chain reaction: A real-time detection approach

✍ Wei Wang; Hai-Bin Wang; Zhi-Xin Li; Zeng-Yuan Guo 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 152 KB

## Abstract In the miniaturization of biochemical analysis systems, biocompatibility of the microfabricated material is a key feature to be considered. A clear insight into interactions between biological reagents and microchip materials will help to build more robust functional bio‐microelectromec