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A simple method for the detection and genotyping of high-risk human papillomavirus using seminested polymerase chain reaction and reverse hybridization

✍ Scribed by Hao Lin; Jau-Sung Moh; Yu-Che Ou; Shu-Yun Shen; Yi-Min Tsai; Chan-Chao ChangChien; Jacqueline M. Liu; Yen-Ying Ma


Book ID
118506616
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
270 KB
Volume
96
Category
Article
ISSN
0090-8258

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## Abstract The ability to detect the presence of human pap‐illomavirus (HPV)‐DNA sequences in urine was evaluated using polymerase chain reaction (PCR). DMA was purified and extracted from urine samples, and subjected to 40 cycles of amplification using the consensus primer pair MY11 and MY09. Coa