A novel scale-up method for mammalian cell culture in packed-bed bioreactor
β Scribed by Chunshui Cong; Yu Chang; Jixian Deng; Chengzu Xiao; Zhiguo Su
- Book ID
- 110292019
- Publisher
- Springer Netherlands
- Year
- 2001
- Tongue
- English
- Weight
- 71 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0141-5492
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## Abstract We have validated our previously described model for scaleβup of packedβbed solidβstate fermenters (Weber et al., 1999) with experiments in an adiabatic 15βdm^3^ packedβbed reactor, using the fungi __Coniothyrium minitans__ and __Aspergillus oryzae__. Effects of temperature on respirati
The increasing demand for products from mammalian cells has prompted the authors to develop a new type of bioreactor. Its significant features include the supply of oxygen, homogeneous distribution of microcarrier suspensions and process control. Media with high protein contents, required for mammal