## Abstract This case study focuses on the scale‐up of a Sp2/0 mouse myeloma cell line based fed‐batch bioreactor process, from the initial 3‐L bench scale to the 2,500‐L scale. A stepwise scale‐up strategy that involved several intermediate steps in increasing the bioreactor volume was adopted to
Scale-up analysis for a CHO cell culture process in large-scale bioreactors
✍ Scribed by Zizhuo Xing; Brian M. Kenty; Zheng Jian Li; Steven S. Lee
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 309 KB
- Volume
- 103
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The complete yeast genome contains a large number of genes of unknown biological function. Simple, rapid and reliable specific screens are valuable tools in exploring gene function via systematic phenotypic analysis of large mutant collections. This report provides a new approach for monitoring chan
## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p
## Abstract We have validated our previously described model for scale‐up of packed‐bed solid‐state fermenters (Weber et al., 1999) with experiments in an adiabatic 15‐dm^3^ packed‐bed reactor, using the fungi __Coniothyrium minitans__ and __Aspergillus oryzae__. Effects of temperature on respirati