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A novel procedure for separating small peptides on polyacrylamide gels

โœ Scribed by Kwabena Sarfo; Greg B. G. Moorhead; Raymond J. Turner

Book ID
111597620
Publisher
Springer Netherlands
Year
2003
Tongue
English
Weight
128 KB
Volume
10
Category
Article
ISSN
1573-3149

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An electrophoretic system for separating, with high resolution, peptides 25-250 residues in length is described. The peptides are stacked by discontinuous electrophoresis to form very sharp bands at the origin. They are then separated on a matrix of 20% polyacrylamide, 8 M urea, and 0.1% dodecyl sul

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PAGE 3 systems capable of separating peptides of M r below 10,000 have been reported. Most of these methods have used separation gels with urea (1-4), high concentrated and crosslinked polyacrylamide (5), or a steep polyacrylamide gradient (6, 7) in order to achieve successful resolution of the pept