A one-step, low background Coomassie staining procedure for polyacrylamide gels
β Scribed by Bradley D. Zehr; Thomas J. Savin; Robert E. Hall
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 368 KB
- Volume
- 182
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A new staining procedure for proteins in polyacrylamide gels has been developed. This procedure, utilizing Coomassie brilliant blue G250, is rapid (as quick as 30 min) and simple to perform, requires little or no destaining, and has a sensitivity of a least 1 pg of protein per band. It can be used t
A procedure which allows quantitative determination of the amino acid composition of stained proteins separated by sodium dodecyl sulfate (SDS)-gel electrophoresis using ninhydrin is described. The Coomassie-protein-SDS complex extracted from the gel is dialyzed, lyophylized, and subjected to amino