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A discontinuous electrophoretic system for separating peptides on polyacrylamide gels

✍ Scribed by Jack Kyte; Henry Rodriguez

Book ID
102986888
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
899 KB
Volume
133
Category
Article
ISSN
0003-2697

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✦ Synopsis

An electrophoretic system for separating, with high resolution, peptides 25-250 residues in length is described. The peptides are stacked by discontinuous electrophoresis to form very sharp bands at the origin. They are then separated on a matrix of 20% polyacrylamide, 8 M urea, and 0.1% dodecyl sulfate. Through this combination, high resolution and clean separation, based on polymer length, are achieved.

πŸ“œ SIMILAR VOLUMES

Polyacrylamide Gel Electrophoresis witho
Polyacrylamide Gel Electrophoresis without a Stacking Gel: Application for Separation of Peptides
✍ Sung-Kun Yim; Taeho Ahn; Joon-Sik Kim; Chul-Ho Yun πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 61 KB

PAGE 3 systems capable of separating peptides of M r below 10,000 have been reported. Most of these methods have used separation gels with urea (1-4), high concentrated and crosslinked polyacrylamide (5), or a steep polyacrylamide gradient (6, 7) in order to achieve successful resolution of the pept